nheks  (ATCC)

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: Plant extract mixture restores gene expression of skin barrier molecules filaggrin and loricrin in the 2D psoriasis model. CaCl 2 -differentiated NHEKs were left unstimulated (control) or stimulated with a psoriasis-like cytokine mixture (IL-1β, IL-17A, IL-22 and TNF-alpha, each 10 ng/mL) either in the absence (Psoriasis) or in the presence of the plant extract (Psoriasis + Extract) for 21 h. Gene expression levels of ( a ) FLG , ( b ) LOR and ( c ) IVL were determined by real-time PCR. Statistical significance was tested by a, c) one-way ANOVA with subsequent Sidak’s multiple comparison test or b) Kruskal-Wallis test with subsequent Dunn’s multiple comparisons test ( n = 12 * p < 0.05; ** p < 0.01; *** p < 0.001; ns = not significant).

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Gene Expression, Control, Real-time Polymerase Chain Reaction, Comparison

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: Plant extract mixture downregulates inflammatory markers in the 2D psoriasis model. CaCl 2 -differentiated NHEKs were stimulated as described in Fig. . Gene expression levels of ( a ) IL1A , ( b ) IL1B, ( c ) IL1RN , ( d ) CXCL8 , ( e ) TNFA , ( f ) IL17C , ( g ) IL36G , ( h ) CSF2 , ( i ) VEGFA were measured. Statistical significance was tested by a, b, c, e, h) one-way ANOVA with subsequent Sidak’s multiple comparison test or d, f, g) Kruskal-Wallis test with subsequent Dunn’s multiple comparisons test ( n = 12, * p < 0.05; ** p < 0.01; *** p < 0.001; ns = not significant). Protein expression levels of ( j ) IL-8 and k) TNFα were measured in the supernatants of the cells by ELISA. Values for unstimulated control and plant extract-treated cells were below the detection limit of 31,3 pg/mL (IL-8) or 62,5 pg/mL (TNFα) and not detectable; therefore, no statistical analysis was performed. n.d.= non-detectable.

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Gene Expression, Comparison, Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: Plant extract mixture lowers upregulated antimicrobial peptide expression in the 2D psoriasis model. CaCl 2 -differentiated NHEKs were stimulated as described in Fig. . Gene expression levels of ( a ) S100A7 , ( c ) DEFB4A and ( e ) DEFB103A were measured. Statistical significance was tested by ( a , c ) one-way ANOVA with subsequent Sidak’s multiple comparison test or ( e ) Kruskal-Wallis test with subsequent Dunn’s multiple comparisons test ( n = 12). Protein expression levels of ( b ) psoriasin and ( d ) hBD2 were measured in the supernatants of the cells by ELISA. Statistical significance was tested by b) Kruskal-Wallis test with subsequent Dunn’s multiple comparisons test ( n = 12) or d) one-way ANOVA with subsequent Sidak’s multiple comparison test ( n = 6, * p < 0.05; ** p < 0.01; *** p < 0.001; ns = not significant).

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Expressing, Gene Expression, Comparison, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: Plant extract mixture reduces NFKBIZ and NFKBIA gene expression and IκBζ protein levels. CaCl 2 -differentiated NHEKs were stimulated as described in Fig. . Gene expression levels of ( a ) NFKBIZ and ( c ) NFKBIA were measured and statistical significance was tested by one-way ANOVA with subsequent Sidak’s multiple comparisons test ( n = 12; * p < 0.05; ** p < 0.01; *** p < 0.001; ns = not significant). ( b ) Protein expression levels of IκBζ were assessed by western blot using an IκBζ antibody. Detection of pan-actin serves as a loading control. Uncropped blots are shown in Supplementary Figure .

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Gene Expression, Expressing, Western Blot, Control

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: Plant extract mixture activates the AhR in the 2D psoriasis model. ( a ) CaCl 2 -differentiated NHEKs were stimulated as described in Fig. . Gene expression levels of CYP1A1 were measured and statistical significance was tested by Kruskal-Wallis test with subsequent Dunn’s multiple comparisons test ( n = 12). ( b ) To determine AhR activation, NHEKs were transfected with the pGudLuc6.1 plasmid containing firefly luciferase which expression depends on AhR activation and the pGL4.74 [ hRLuc /TK] reference plasmid containing renilla luciferase. One day after transfection, cells were stimulated as described in ( a ). After cell lysis, activation of AhR was determined by measurement of relative luciferase activities. Statistical significance was tested by one-way ANOVA with subsequent Sidak’s multiple comparison test ( n = 12; ** p < 0.01; *** p < 0.001; ns = not significant).

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Gene Expression, Activation Assay, Transfection, Plasmid Preparation, Luciferase, Expressing, Lysis, Comparison

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: Downregulation of AhR inhibits the filaggrin-inducing effects but not the anti-inflammatory effects of the plant extract in the 2D psoriasis model. NHEKs were transfected with control or AhR siRNA and stimulated as described in Fig. . Gene expression levels of ( a ) CYP1A1 , ( b ) FLG , ( c ) NFKBIZ , ( d ) TNFA , ( e ) IL36G ( f ) CXCL8 and ( g ) DEFB4A were measured. Statistical significance was determined by ( a – f ) one-way ANOVA with subsequent Sidak’s multiple comparison test or ( c ) Kruskal-Wallis with subsequent Dunn’s multiple comparison test (n = 9; * p < 0.05; ** p < 0.01; *** p 0.001; ns = not significant).

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Transfection, Control, Gene Expression, Comparison

Journal: Scientific Reports

Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model

doi: 10.1038/s41598-026-50000-8

Figure Lengend Snippet: The plant extract mixture exhibits antioxidant effects in the 2D psoriasis model. CaCl 2 -differentiated NHEKs were stimulated as described in Fig. . ( a ) Gene expression levels of NQO1 were measured and statistical significance was tested by one-way ANOVA with subsequent Sidak’s multiple comparisons test ( n = 12). ( b ) Intracellular reactive oxygen species were measured by DCFDA-based assay. The mean fluorescence value of the control cells was set to 100%, and the relative intracellular ROS levels of the other samples were calculated as percentages of the control. Statistical significance was tested by Kruskal-Wallis test with subsequent Dunn’s multiple comparison test ( n = 12, * p < 0.05; ** p < 0.01; *** p < 0.001; ns = not significant). ( c ) NHEKs were transfected and stimulated as described in Fig. . Gene expression levels of NQO1 were measured and statistical significance was determined by one-way ANOVA with subsequent Sidak’s multiple comparison test ( n = 9; * p < 0.05; ns = not significant).

Article Snippet: For the 2D psoriasis model, primary normal human epidermal keratinocytes (NHEKs, pooled from four donors, PromoCell, Heidelberg Germany), used at passage 4, were seeded in a 24-well plate in Derma Life Complete medium (CellSystems, Troisdorf, Germany).

Techniques: Plant Extract, Gene Expression, Fluorescence, Control, Comparison, Transfection

Journal: GeoHealth

Article Title: Effects of Ozone‐Depleting Substances on Ultraviolet Radiation and Skin Cancer Rates in Australia and the United States of America

doi: 10.1029/2024GH001283

Figure Lengend Snippet: Increases in annual UV radiation and UV‐related maladies due to ODS‐derived ozone‐depletion, estimated by the AHEF. Australia (solid lines), USA (dashed lines). UV exposure at 35° latitude (black). Squamous cell carcinoma (SCC) cases (light green), basal cell carcinoma (BCC) cases (dark green), melanoma cases (MC, magenta), melanoma deaths (dark red), keratinocyte carcinoma (KC) deaths (teal), cataract cases (light red). Combined KC case rate increases (not plotted) peak at 2.9% (AUS) and 3.2% (USA). Malady rates are age standardized.

Article Snippet: Primary keratinocyte cancer incidence can be accessed from Staples et al. ( , ).

Techniques: Derivative Assay

Journal: GeoHealth

Article Title: Effects of Ozone‐Depleting Substances on Ultraviolet Radiation and Skin Cancer Rates in Australia and the United States of America

doi: 10.1029/2024GH001283

Figure Lengend Snippet: Model‐data comparison for annual keratinocyte cancer incidence in Australia. Symbols, data; dashed lines, model calculations. Dark blue, male; pink, female. Open diamonds, “estimated AUS‐wide cases” from Table 2 of Staples et al. ; filled triangles with solid vertical lines, product of age‐standardized rates (with 95% confidence intervals) from Table 4 of Staples et al. and Australia population. Open circles, sum of basal cell carcinoma (BCC) + squamous cell carcinoma (SCC) (Pandeya et al., ); stars, net keratinocyte carcinoma (KC) cases (Pandeya et al. . Note that the model calculations are initialized independently of the data plotted here.

Article Snippet: Primary keratinocyte cancer incidence can be accessed from Staples et al. ( , ).

Techniques: Comparison

Journal: GeoHealth

Article Title: Effects of Ozone‐Depleting Substances on Ultraviolet Radiation and Skin Cancer Rates in Australia and the United States of America

doi: 10.1029/2024GH001283

Figure Lengend Snippet: Comparison of modeled and reported data for Australia for (a) keratinocyte deaths, (b) melanoma cases and (c) melanoma deaths. Solid lines, data (AIHW, , ); dashed lines, model. Gray bar, date range of baseline data. Dark blue, male; pink, female.

Article Snippet: Primary keratinocyte cancer incidence can be accessed from Staples et al. ( , ).

Techniques: Comparison

Journal: International Journal of Molecular Sciences

Article Title: Activity and Biocompatibility Evaluation of Enzybiotic Compositions Formulated with Azotobacter vinelandii Alginate for Topical Use

doi: 10.3390/ijms27093856

Figure Lengend Snippet: HaCaT cell viability in the presence of gel components and the composition. bALG, bacterial sodium alginate; bALG-Lys, composition of sodium alginate (2% initial concentration) and LysSi3-LK (0.5 mg/mL initial concentration). The mean values for three replicates are shown for all groups of samples (± SD). *— p < 0.05, ****— p < 0.0001 compared to bALG-Lys group, two-way ANOVA.

Article Snippet: HaCaT keratinocytes (ATCC PCS-200-011) were seeded into a 96-well plate for adherent cultures at a density of 20,000 cells/well.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Activity and Biocompatibility Evaluation of Enzybiotic Compositions Formulated with Azotobacter vinelandii Alginate for Topical Use

doi: 10.3390/ijms27093856

Figure Lengend Snippet: Results of the migration assay of HaCaT cells with the investigated preparations within 48 h. Scratch closure area, which was occupied by the cells for: ( a ) Bacterial sodium alginate gel samples; ( b ) Bacterial calcium alginate hydrogel samples. Data are presented as mean values ± SD. ( c ) Bright field light microscopy (scale bar, 200 μm). Na-ALG, bacterial sodium alginate gels; Ca-ALG, bacterial calcium alginate hydrogels; Lys, endolysin LysSi3-LK. **— p < 0.01, ***— p < 0.001 compared to bALG group, one-way ANOVA.

Article Snippet: HaCaT keratinocytes (ATCC PCS-200-011) were seeded into a 96-well plate for adherent cultures at a density of 20,000 cells/well.

Techniques: Migration, Light Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Activity and Biocompatibility Evaluation of Enzybiotic Compositions Formulated with Azotobacter vinelandii Alginate for Topical Use

doi: 10.3390/ijms27093856

Figure Lengend Snippet: HaCaT cells viability dynamics in the biocompatibility assay: ( a ) Viable cells percent, mean values are presented ± SD; ( b ) Fluorescence microscopy of HaCaT incorporated into bALG calcium-alginate films with addition of the endolysin LysSi3-LK in 0.25 and 0.5 μg/mL concentrations. *— p < 0.05; **— p < 0.01, two-way ANOVA.

Article Snippet: HaCaT keratinocytes (ATCC PCS-200-011) were seeded into a 96-well plate for adherent cultures at a density of 20,000 cells/well.

Techniques: Fluorescence, Microscopy